{"id":4150,"date":"2022-01-17T10:15:41","date_gmt":"2022-01-17T02:15:41","guid":{"rendered":"http:\/\/www.tinyblog.cn\/?p=4150"},"modified":"2023-03-22T13:53:59","modified_gmt":"2023-03-22T05:53:59","slug":"praesto-jetted-a50-hiph","status":"publish","type":"post","link":"http:\/\/www.tinyblog.cn\/?p=4150","title":{"rendered":"Praesto\u00ae Jetted A50 HipH"},"content":{"rendered":"

\u70b9\u51fb\u6570\uff1a621<\/p>\t\t

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\u901a\u8fc7\u4e0eRepligen\u5408\u4f5c\uff08\u91c7\u7528NGL – Impact\u00ae A Hi pH\u914d\u57fa\uff09\uff0cPraesto Jetted A50 HipH\u662fPurolite\u5728\u4eb2\u548c\u5c42\u6790\u586b\u6599\u4e0a\u6700\u65b0\u7684\u6280\u672f\u521b\u65b0\u3002Purolite\u5168\u65b0\u7684\u86cb\u767dA\u586b\u6599\u89e3\u51b3\u4e86\u90a3\u4e9b\u5bf9pH\u654f\u611f\u7684\u86cb\u767d\u56e0\u4f20\u7edf\u86cb\u767d\u586b\u6599\u9700\u8981\u4f4epH\u6d17\u8131\u7684\u56f0\u6270\u3002Jetted A50 HipH\u53ef\u4ee5\u7528\u4e8e\u90a3\u4e9b\u654f\u611f\u7684\u86cb\u767d\u3002<\/p>

\u76f8\u8f83\u4e8e\u4f20\u7edf\u7684\u5355\u514b\u9686\u6297\u4f53\u7eaf\u5316\u5de5\u827a\u53bb\u9664\u6742\u8d28\uff0c\u5229\u7528Praesto\u55b7\u5c04A50 HipH\u72ec\u7279\u7684\u6d17\u8131\u7279\u6027\u53ef\u4ee5\u5f97\u5230\u663e\u8457\u7684\u63d0\u5347\u3002<\/span><\/p><\/div><\/div>

\u00a0Praesto Jetted A50 HipH\u7684\u88f8\u7403\u662f\u4f7f\u7528\u4e13\u5229\u7684\u55b7\u5c04\u6280\u672f\u751f\u4ea7\u7684\uff0c\u4e00\u79cd\u521b\u65b0\u7684\u5de5\u827a\u53ef\u4ee5\u751f\u4ea7\u7c92\u5f84\u5206\u5e03\u975e\u5e38\u7a84\u7684\u5747\u4e00\u7c92\u5f84\u7684\u743c\u8102\u7cd6\u88f8\u7403\u3002\u8fd9\u4e9b\u5747\u4e00\u7c92\u5f84\u7684\u88f8\u7403\u5bf9\u6bd4\u5e38\u89c4\u7684\u586b\u6599\u6709\u7740\u4f18\u5f02\u7684\u6027\u80fd\u8868\u73b0\u3002<\/div>
\u00a0<\/div><\/div>

\u6709\u5173\u6211\u4eec\u7684Protein A\u586b\u6599\u7684\u5e38\u89c1\u95ee\u9898\uff0c\u8bf7\u8bbf\u95ee\u6211\u4eec\u7684FAQs<\/a>\u9875\u9762\u3002<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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PRINCIPAL APPLICATIONS<\/strong><\/span><\/p>

  • MAb Purification<\/li><\/ul><\/td>

REGULATORY APPROVALS<\/strong><\/span><\/p>

  • Manufactured under cGMP conditions<\/li><\/ul><\/td><\/tr>

ADVANTAGES<\/strong><\/span><\/p>

  • Manufactured using patented Jetting technique
    Uniform particle size<\/li>
  • Religen ligand NGL-Impact\u00ae A Hi pH<\/li>
  • Dynamic binding capacity up to ~60 mg\/hIgG\/ml resin
    Alkaline stable in 0.1 M NaOH for over 100 cycles<\/li>
  • Enhanced pressure\/flow performance \u2013 Flows are up to 250 cm\/h in a 60 x 20 cm column<\/li>
  • Manufactured with reduced environmental impact<\/li><\/ul><\/td>

TYPICAL PACKAGING<\/strong><\/span><\/p>

  • Bulk Resin<\/li>
  • Production-Scale OPUS\u00ae Columns<\/li>
  • OPUS\u00ae Robocolumns\u00ae<\/li>
  • OPUS\u00ae MiniChrom Columns<\/li>
  • HT Columns<\/li><\/ul><\/td><\/tr><\/tbody><\/table>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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    TYPICAL PHYSICAL & CHEMICAL CHARACTERISTICS<\/span>:<\/span><\/strong><\/span><\/td>\u00a0<\/td><\/tr>
    Polymer Structure<\/strong><\/td>Highly cross linked agarose<\/td>\u00a0<\/td><\/tr>
    Appearance<\/strong><\/td>Spherical, uniform size beads supplied in 20% ethanol slurry. On request 2% benzylalcohol<\/td><\/tr>
    Functional Group<\/strong><\/td>Protein A<\/td>\u00a0<\/td><\/tr>
    Average Particle Size (d50\u1d65), \u03bcm<\/strong><\/td>50 \u00b5m<\/td>\u00a0<\/td><\/tr>
    Dynamic Binding Capacity (min.)- 3 minutes residence time<\/strong><\/td>Up to ~60 mg hIgG\/ml resin<\/td>\u00a0<\/td><\/tr>
    Pressure\/flow (min.) – at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar)<\/strong><\/td>Up to 250 cm\/h (20 x 60 cm column)<\/td>\u00a0<\/td><\/tr>
    pH stability, CIP (short term)<\/strong><\/td>2 – 14<\/td>\u00a0<\/td><\/tr>
    pH stability, working range<\/strong><\/td>3 – 12<\/td>\u00a0<\/td><\/tr>
    Recommended Storage<\/strong><\/td>2 – 8 \u00b0C<\/td>\u00a0<\/td><\/tr>
    Recommended Storage<\/strong><\/td>20% ethanol<\/td>\u00a0<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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    Praesto\u00ae Jetting technology creates enhanced Protein A agarose resins for bioprocessing of recombinant proteins and monoclonal antibodies (mAbs)<\/strong><\/span><\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    Peformance data<\/strong><\/span><\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    Figure 2a and 2b<\/span> Breakthrough capacity for polyclonal immunoglobulin G (hIgG) and an IgG1 monoclonal antibody (mAb 1) of Praesto Jetted A50 HipH, Praesto Jetted A50 and MabSelect SuRe LX.<\/strong><\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    Praesto Jetted HipH A exhibits excellent capacity for polyclonal human immunoglobulin G and an IgG1 subclass monoclonal antibody. Excellent capacity and selectivity are available for molecules previously sensitive to low pH elution. An equivalent capacity to the market leading protein A resin is achievable using Praesto Jetted HipH A.<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    Figure 3<\/span> Sodium hydroxide stability of Praesto Jetted A50 HipH. Tested with hIgG at selected exposure intervals.<\/strong><\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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    Sodium hydroxide (NaOH) is commonly used in
    bioprocessing as an industry standard for cleaning in place, as such it is increasingly important for capture resins to be extremely alkaline stable. Sodium hydroxide exhibits high efficiency in removing bound proteins, nucleic acids, and lipids from bioprocess resins, alleviating the risk of fouling on heavily burdened protein A columns. Praesto Jetted HipH A50 has been shown to be alkaline stable for over 100 hours exposure to 0.1 M NaOH.<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    Process performance<\/strong><\/span><\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    Purification performance of Praesto Jetted A50 HipH using elution conditions from acidic to mild was investigated. Quantitative elution was achieved up to pH 5.0 using a clarified harvest of an IgG1 subclass monoclonal antibody with recovery levels greater than 95% and elution within 2.5 column volumes. Leached Protein A levels determined to be similar to that of Praesto Jetted A50.<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    Process performance – Customer evaluation<\/strong><\/span><\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    Customer evaluations have shown the effect of using the unique properties of Praesto Jetted A50 HipH to elute at much higher pH when compared to conventional protein A chromatography. Two mAbs were tested, listed as mAb 2 and mAb 3.
    Minimal impact on purity is observed by increasing the elution pH of the buffer to elute mAb 2 and mAb 3. The log reduction in host cell proteins and separation of the target molecule from residual DNA is markedly improved by eluting at a higher pH.<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    \n\t\t\t\t\t\t\t\t\t\t\t\t\"\"\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

    Purolite\u5168\u65b0\u7684\u86cb\u767dA\u586b\u6599\u89e3\u51b3\u4e86\u90a3\u4e9b\u5bf9pH\u654f\u611f\u7684\u86cb\u767d\u56e0\u4f20\u7edf\u86cb\u767d\u586b\u6599\u9700\u8981\u4f4epH\u6d17\u8131\u7684\u56f0\u6270\u3002Jetted A50 HipH\u53ef\u4ee5\u7528\u4e8e\u90a3\u4e9b\u654f\u611f\u7684\u86cb\u767d\u3002<\/p>\n","protected":false},"author":1,"featured_media":4199,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[30],"tags":[],"_links":{"self":[{"href":"http:\/\/www.tinyblog.cn\/index.php?rest_route=\/wp\/v2\/posts\/4150"}],"collection":[{"href":"http:\/\/www.tinyblog.cn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.tinyblog.cn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.tinyblog.cn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.tinyblog.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4150"}],"version-history":[{"count":0,"href":"http:\/\/www.tinyblog.cn\/index.php?rest_route=\/wp\/v2\/posts\/4150\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.tinyblog.cn\/index.php?rest_route=\/wp\/v2\/media\/4199"}],"wp:attachment":[{"href":"http:\/\/www.tinyblog.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4150"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.tinyblog.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4150"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.tinyblog.cn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4150"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}